Descripción: “...Sample size will be obtained by convenience, according to the number of soft contact lenses users attended at the Fundación Universitaria del Area Andina over a period of twelve months, the independent variables will be the polymerase chain reaction and the conventional methods (culture plate agar, confirmative test and antibiogram), to determine the presence, antibiotic resistence and to decrease identification time of P. aeruginosa and S. aureus. ...”