Descripción: “...Semen samples were collected by digital manipulation  of penis, collecting only the  second fraction  of the ejaculate. Each aliquot was diluted in proportions 1:2, 1:4 or 1:8 until total  volume of 0.5  ml, using the extenders  TRIS- citric  acidegg yolk or TRISSodium citrateegg yolk, with 1,3 or 1,6 g of fructose. ...”

Descripción: “...The seminal characteristics of 19 samples were registered and aliquots of 250 mL were diluted in two different solutions with 0, 5, 10  or 15%  of DMSO, then stored low refrigeration and the motility monitored at 0, 1, 2, 4, 8, 16, 24, 32, 40 and 48 h. ...”

Descripción: “...The latter was included in three  aliquots in intervals of 15 min. The semen was packaged in  straws, each containing 0.5  ml., and cryopreserved in a programmable  freezer  CL-8800.  ...”