Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed
ASBTRACT: The Murciano-levantina cattle from the Spanish South-East belongs to a bovine endangered breed under the special protection of FAO (WWL-DAD:3)*. Their characteristics of rusticity, longe- vity, docility and disease resistance make interesting the recovery of the breed. The objective of the present work was to deter mine the efficiency of in vitro bovine embryo production using oocytes collected from post mortem ovaries fertilized with frozen thaw sperm obtained either from commercial or Murciano-levantina bulls. Cumulus oocyte complexes were matured for 24 h in TCM199 with 10% FBS, 2mM L-glutamine, 0.2 mM sodium pyruvate, 0.3 µg/mL LH and µg/mL FSH. Oocytes presumed matured were fertilized with Percoll-prepared sp... Ver más
0121-3709
2011-2629
8
2004-01-01
25
25
http://purl.org/coar/access_right/c_abf2
info:eu-repo/semantics/openAccess
Orinoquia - 2014
